cd11b antibody, anti-human, reafinity (Miltenyi Biotec)
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Cd11b Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b/CD11b+Antibody%2C+anti-human%2C+REAfinity/custom%40130-135-497%4042502392
Average 93 stars, based on 6 article reviews
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Magnetic Cell Separation:Article Title: SiglecF-Expressing Neutrophils Differentiate from Mature Neutrophils Locally in the Heart after Myocardial Infarction. Article Snippet: .. The cell pellet was resuspended in 1 mL of ice-cold MACS Rinsing Buffer (Miltenyi Biotec) containing fluorescently labeled antibodies specific for Labeling:Article Title: SiglecF-Expressing Neutrophils Differentiate from Mature Neutrophils Locally in the Heart after Myocardial Infarction. Article Snippet: .. The cell pellet was resuspended in 1 mL of ice-cold MACS Rinsing Buffer (Miltenyi Biotec) containing fluorescently labeled antibodies specific for Immunopeptidomics:Article Title: A targetable dependency on nonsense-mediated decay for proteostasis and immune control in small cell lung cancer Article Snippet: Flow cytometry data was analyzed with Flowjo software. .. Human primary antibodies used were B2M (Molecular probes #A15770), MHC-ABC (Invitrogen #11998342), CD45 (Miltenyi #130110636), CD3 (Miltenyi #130113142), CD4 (Biolegend #357410), CD8 (Miltenyi #130110678), CD56 (Miltenyi #130113310), Flow Cytometry:Article Title: A novel Chimeric Antigen Receptor (CAR) - strategy to target EGFR VIII -mutated glioblastoma cells via macrophages Article Snippet: After culture for 48 h at 37 °C, 5% CO 2 , adherent cells were detached using StemProTM AccutaseTM (Gibco, Cat#: A1110501) for 10 min and analyzed by FACS to evaluate the phenotypic markers of M1 or M2 macrophages. .. Flow cytometry was performed by using classical myeloid markers such as FACS:Article Title: Maternal Obesity Programs Adipogenic Commitment in Neonatal Mesenchymal Stem Cells: A Link to Redox‐Dependent FOXO1 Signaling Article Snippet: .. Surface markers were characterized according to the International Society of Cell Therapy criteria (Dominici et al. 2006) Briefly, cells were detached with trypsin and centrifuged at 2500 g for 3 min. After this, 1 × 10 6 cells were resuspended in 100 μL of FACS buffer (2% FCS in DPBS) in FACS tubes (Falcon, Corning) and incubated for 30 min in the dark with antibodies for CD73 (APC, 1:50, Miltenyi Biotec), CD90 (PE, 1:50, Miltenyi Biotec), CD105 (BV421, 1:20, BioLegend), CD34 (PE‐Dazzle 594, 1:20, Biolegend), CD45 (BV785, 1:20, BioLegend), and Incubation:Article Title: Maternal Obesity Programs Adipogenic Commitment in Neonatal Mesenchymal Stem Cells: A Link to Redox‐Dependent FOXO1 Signaling Article Snippet: .. Surface markers were characterized according to the International Society of Cell Therapy criteria (Dominici et al. 2006) Briefly, cells were detached with trypsin and centrifuged at 2500 g for 3 min. After this, 1 × 10 6 cells were resuspended in 100 μL of FACS buffer (2% FCS in DPBS) in FACS tubes (Falcon, Corning) and incubated for 30 min in the dark with antibodies for CD73 (APC, 1:50, Miltenyi Biotec), CD90 (PE, 1:50, Miltenyi Biotec), CD105 (BV421, 1:20, BioLegend), CD34 (PE‐Dazzle 594, 1:20, Biolegend), CD45 (BV785, 1:20, BioLegend), and Isolation:Article Title: Increased Constitutive Interferon‐β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8 + , CD11b + and CD11c + cells with TRI reagent (Sigma‐Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse‐transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8 + , Article Title: Increased Constitutive Interferon-β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein. Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8+ , CD11b+ and CD11c+ cells with TRI reagent (Sigma-Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse-transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8+ , Selection:Article Title: Increased Constitutive Interferon‐β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8 + , CD11b + and CD11c + cells with TRI reagent (Sigma‐Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse‐transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8 + , Article Title: Increased Constitutive Interferon-β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein. Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8+ , CD11b+ and CD11c+ cells with TRI reagent (Sigma-Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse-transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8+ , |
