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cd11b antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd11b antibody, anti-human, reafinity
    Cd11b Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b/CD11b+Antibody%2C+anti-human%2C+REAfinity/custom%40130-135-497%4042502392
    Average 93 stars, based on 6 article reviews
    cd11b antibody, anti-human, reafinity - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Magnetic Cell Separation:

    Article Title: SiglecF-Expressing Neutrophils Differentiate from Mature Neutrophils Locally in the Heart after Myocardial Infarction.
    Article Snippet: .. The cell pellet was resuspended in 1 mL of ice-cold MACS Rinsing Buffer (Miltenyi Biotec) containing fluorescently labeled antibodies specific for CD11b, Ly6G, and CD101. ..

    Labeling:

    Article Title: SiglecF-Expressing Neutrophils Differentiate from Mature Neutrophils Locally in the Heart after Myocardial Infarction.
    Article Snippet: .. The cell pellet was resuspended in 1 mL of ice-cold MACS Rinsing Buffer (Miltenyi Biotec) containing fluorescently labeled antibodies specific for CD11b, Ly6G, and CD101. ..

    Immunopeptidomics:

    Article Title: A targetable dependency on nonsense-mediated decay for proteostasis and immune control in small cell lung cancer
    Article Snippet: Flow cytometry data was analyzed with Flowjo software. .. Human primary antibodies used were B2M (Molecular probes #A15770), MHC-ABC (Invitrogen #11998342), CD45 (Miltenyi #130110636), CD3 (Miltenyi #130113142), CD4 (Biolegend #357410), CD8 (Miltenyi #130110678), CD56 (Miltenyi #130113310), CD11b (Miltenyi #130110556). .. Murine primary antibodies used were CD45 (Miltenyi #130110665), CD3 (Miltenyi #130119793), Nkp46 (BD Biosciences #3122669), CD19 (Miltenyi #130112037), CD11b (Miltenyi #1301138063), CD14 (Miltenyi #130115559), H2-Kb (Miltenyi #130115586), IFN-γ (ThermoFisher #48731182), KI-67 (Miltenyi #130120418).

    Flow Cytometry:

    Article Title: A novel Chimeric Antigen Receptor (CAR) - strategy to target EGFR VIII -mutated glioblastoma cells via macrophages
    Article Snippet: After culture for 48 h at 37 °C, 5% CO 2 , adherent cells were detached using StemProTM AccutaseTM (Gibco, Cat#: A1110501) for 10 min and analyzed by FACS to evaluate the phenotypic markers of M1 or M2 macrophages. .. Flow cytometry was performed by using classical myeloid markers such as CD11b (Miltenyi Biotec Cat#: 130-110-558), HLA-DR(MHC-II) (Miltenyi Biotec Cat#: 130-111-795) as well as M1 and M2-skewed markers like CD80(Miltenyi Biotec Cat#: 130-117-719), CD86(Miltenyi Biotec Cat#: 130-116-164), CD206(Miltenyi Biotec Cat#: 130-133-375) and CD163(Miltenyi Biotec Cat#: 130-112-129). ..

    FACS:

    Article Title: Maternal Obesity Programs Adipogenic Commitment in Neonatal Mesenchymal Stem Cells: A Link to Redox‐Dependent FOXO1 Signaling
    Article Snippet: .. Surface markers were characterized according to the International Society of Cell Therapy criteria (Dominici et al. 2006) Briefly, cells were detached with trypsin and centrifuged at 2500 g for 3 min. After this, 1 × 10 6 cells were resuspended in 100 μL of FACS buffer (2% FCS in DPBS) in FACS tubes (Falcon, Corning) and incubated for 30 min in the dark with antibodies for CD73 (APC, 1:50, Miltenyi Biotec), CD90 (PE, 1:50, Miltenyi Biotec), CD105 (BV421, 1:20, BioLegend), CD34 (PE‐Dazzle 594, 1:20, Biolegend), CD45 (BV785, 1:20, BioLegend), and CD11b (APC‐Vio770, 1:50, Miltenyi Biotec). ..

    Incubation:

    Article Title: Maternal Obesity Programs Adipogenic Commitment in Neonatal Mesenchymal Stem Cells: A Link to Redox‐Dependent FOXO1 Signaling
    Article Snippet: .. Surface markers were characterized according to the International Society of Cell Therapy criteria (Dominici et al. 2006) Briefly, cells were detached with trypsin and centrifuged at 2500 g for 3 min. After this, 1 × 10 6 cells were resuspended in 100 μL of FACS buffer (2% FCS in DPBS) in FACS tubes (Falcon, Corning) and incubated for 30 min in the dark with antibodies for CD73 (APC, 1:50, Miltenyi Biotec), CD90 (PE, 1:50, Miltenyi Biotec), CD105 (BV421, 1:20, BioLegend), CD34 (PE‐Dazzle 594, 1:20, Biolegend), CD45 (BV785, 1:20, BioLegend), and CD11b (APC‐Vio770, 1:50, Miltenyi Biotec). ..

    Isolation:

    Article Title: Increased Constitutive Interferon‐β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein
    Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8 + , CD11b + and CD11c + cells with TRI reagent (Sigma‐Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse‐transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8 + , CD11b + , and CD11c + cells from spleen were isolated using positive selection with microbeads and magnetized columns (Miltenyi). pDCs were isolated using negative selection with pDC Isolation Kit (Miltenyi), and total RNA was isolated with RNeasy Plus Micro Kit (QIAGEN). .. PCR amplification was performed in a 7500/7500 Fast Real‐Time PCR machine (Applied Biosystems) with Absolute Blue qPCR SYBR low ROX mix (Thermo Fisher).

    Article Title: Increased Constitutive Interferon-β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein.
    Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8+ , CD11b+ and CD11c+ cells with TRI reagent (Sigma-Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse-transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8+ , CD11b+ , and CD11c+ cells from spleen were isolated using positive selection with microbeads and magnetized columns (Miltenyi). pDCs were isolated using negative selection with pDC Isolation Kit (Miltenyi), and total RNA was isolated with RNeasy Plus Micro Kit (QIAGEN). .. PCR amplification was performed in a 7500/7500 Fast Real-Time PCR machine (Applied Biosystems) with Absolute Blue qPCR SYBR low ROX mix (Thermo Fisher).

    Selection:

    Article Title: Increased Constitutive Interferon‐β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein
    Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8 + , CD11b + and CD11c + cells with TRI reagent (Sigma‐Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse‐transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8 + , CD11b + , and CD11c + cells from spleen were isolated using positive selection with microbeads and magnetized columns (Miltenyi). pDCs were isolated using negative selection with pDC Isolation Kit (Miltenyi), and total RNA was isolated with RNeasy Plus Micro Kit (QIAGEN). .. PCR amplification was performed in a 7500/7500 Fast Real‐Time PCR machine (Applied Biosystems) with Absolute Blue qPCR SYBR low ROX mix (Thermo Fisher).

    Article Title: Increased Constitutive Interferon-β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein.
    Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8+ , CD11b+ and CD11c+ cells with TRI reagent (Sigma-Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse-transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8+ , CD11b+ , and CD11c+ cells from spleen were isolated using positive selection with microbeads and magnetized columns (Miltenyi). pDCs were isolated using negative selection with pDC Isolation Kit (Miltenyi), and total RNA was isolated with RNeasy Plus Micro Kit (QIAGEN). .. PCR amplification was performed in a 7500/7500 Fast Real-Time PCR machine (Applied Biosystems) with Absolute Blue qPCR SYBR low ROX mix (Thermo Fisher).



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    Characterization of Res-PD-L1@nmEVs . (A) Schematic illustration of the Res-PD-L1@nmEVs synthesis procedure. (B-D) Representative transmission electron microscopy (TEM) images, dynamic light scattering (DLS) size distributions, and zeta potential measurements of nEVs, PD-L1@mEVs, PD-L1@nmEVs, and Res-PD-L1@nmEVs. (E) PD-L1 expression in PD-L1-overexpressing MSCs (OE-PD-L1) and negative control (NC) MSCs, and <t>CD11b</t> expression in HL60 cells before and after DMSO stimulation, as determined by Western blot. (F) Expression levels of neutrophil membrane markers (CD11b, CXCR2, RAGE, TLR2) and the exosomal marker CD63 in the four EV types. (G) Fluorescence co-localization images of DiO-labeled nEVs (green) and DiL-labeled PD-L1@mEVs (red) after fusion, demonstrating hybrid vesicle formation. (H) Size stability of Res-PD-L1@nmEVs stored at 4 °C and 37 °C for 7 days. (I-K) Binding and neutralization capacity of Res-PD-L1@nmEVs against inflammatory cytokines (TNF-α, IL-6, IL-1β) in vitro. ∗ vs. 0ug/ml; # vs. 100 μg/ml, p < 0.05, n = 5.
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    Image Search Results


    Characterization of Res-PD-L1@nmEVs . (A) Schematic illustration of the Res-PD-L1@nmEVs synthesis procedure. (B-D) Representative transmission electron microscopy (TEM) images, dynamic light scattering (DLS) size distributions, and zeta potential measurements of nEVs, PD-L1@mEVs, PD-L1@nmEVs, and Res-PD-L1@nmEVs. (E) PD-L1 expression in PD-L1-overexpressing MSCs (OE-PD-L1) and negative control (NC) MSCs, and CD11b expression in HL60 cells before and after DMSO stimulation, as determined by Western blot. (F) Expression levels of neutrophil membrane markers (CD11b, CXCR2, RAGE, TLR2) and the exosomal marker CD63 in the four EV types. (G) Fluorescence co-localization images of DiO-labeled nEVs (green) and DiL-labeled PD-L1@mEVs (red) after fusion, demonstrating hybrid vesicle formation. (H) Size stability of Res-PD-L1@nmEVs stored at 4 °C and 37 °C for 7 days. (I-K) Binding and neutralization capacity of Res-PD-L1@nmEVs against inflammatory cytokines (TNF-α, IL-6, IL-1β) in vitro. ∗ vs. 0ug/ml; # vs. 100 μg/ml, p < 0.05, n = 5.

    Journal: Bioactive Materials

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia

    doi: 10.1016/j.bioactmat.2026.03.024

    Figure Lengend Snippet: Characterization of Res-PD-L1@nmEVs . (A) Schematic illustration of the Res-PD-L1@nmEVs synthesis procedure. (B-D) Representative transmission electron microscopy (TEM) images, dynamic light scattering (DLS) size distributions, and zeta potential measurements of nEVs, PD-L1@mEVs, PD-L1@nmEVs, and Res-PD-L1@nmEVs. (E) PD-L1 expression in PD-L1-overexpressing MSCs (OE-PD-L1) and negative control (NC) MSCs, and CD11b expression in HL60 cells before and after DMSO stimulation, as determined by Western blot. (F) Expression levels of neutrophil membrane markers (CD11b, CXCR2, RAGE, TLR2) and the exosomal marker CD63 in the four EV types. (G) Fluorescence co-localization images of DiO-labeled nEVs (green) and DiL-labeled PD-L1@mEVs (red) after fusion, demonstrating hybrid vesicle formation. (H) Size stability of Res-PD-L1@nmEVs stored at 4 °C and 37 °C for 7 days. (I-K) Binding and neutralization capacity of Res-PD-L1@nmEVs against inflammatory cytokines (TNF-α, IL-6, IL-1β) in vitro. ∗ vs. 0ug/ml; # vs. 100 μg/ml, p < 0.05, n = 5.

    Article Snippet: For detecting neutrophil characteristic proteins, the primary antibodies comprised CD11b (HA722075, HUABIO, China), CXCR2 (ER1906-87, HUABIO, China), RAGE (ET1702-27, HUABIO, China), TLR2 (ET1705-92, HUABIO, China), TNFR1(RT1624, HUABIO, China), IL6R ( AWA43078 , Abiowell, China) and the exosome marker CD63 (ET1607-2, HUABIO, China).

    Techniques: Transmission Assay, Electron Microscopy, Zeta Potential Analyzer, Expressing, Negative Control, Western Blot, Membrane, Marker, Fluorescence, Labeling, Binding Assay, Neutralization, In Vitro

    Res-PD-L1@nmEVs Suppresses Neutrophil Activation and Preserves Mitochondrial Integrity via PD-L1 Delivery (A-B) Rats subjected to lung IRI received nebulized administration of different formulations (Res, nEVs, PD-L1@mEVs, PD-L1@nmEVs, or Res-PD-L1@nmEVs) before ischemia and after reperfusion. Lung tissues were collected 2 h post-reperfusion. (A) Representative immunofluorescence images showing the expression and localization of CD11b (green), MPO (red), and PD-1 (yellow) in lung sections across treatment groups. (B) Enlarged view of the IRI group from (A). (C-D) mRNA levels of CD95 (C) and CD206 (D) in lung tissues. (E-F) Levels of myeloperoxidase (MPO) (E) and matrix metalloproteinase-9 (MMP-9) (F) in bronchoalveolar lavage fluid (BALF). (G-I) (G) Representative transmission electron microscopy (TEM) images of lung tissues (scale bar: 2 μm). (H) Proportion of damaged mitochondria. (I) Average number of mitophagic events per cell. (J) Immunofluorescence co-localization of mitochondrial marker TOMM20 (red) and EpCAM (green) in lung tissues (nuclei stained with DAPI, scale bar: 50 μm). (K-L) Protein expression levels of Beclin-1 (K) and LC3 (L) in lung tissues, with insets showing immunofluorescence co-localization of Beclin-1 (green) and LC3 (red) across treatment groups (nuclei stained with DAPI, scale bar: 50 μm). ∗ vs. Sham; # vs. IRI; & vs. IRI + PD-L1@nmEVs, p < 0.05.

    Journal: Bioactive Materials

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia

    doi: 10.1016/j.bioactmat.2026.03.024

    Figure Lengend Snippet: Res-PD-L1@nmEVs Suppresses Neutrophil Activation and Preserves Mitochondrial Integrity via PD-L1 Delivery (A-B) Rats subjected to lung IRI received nebulized administration of different formulations (Res, nEVs, PD-L1@mEVs, PD-L1@nmEVs, or Res-PD-L1@nmEVs) before ischemia and after reperfusion. Lung tissues were collected 2 h post-reperfusion. (A) Representative immunofluorescence images showing the expression and localization of CD11b (green), MPO (red), and PD-1 (yellow) in lung sections across treatment groups. (B) Enlarged view of the IRI group from (A). (C-D) mRNA levels of CD95 (C) and CD206 (D) in lung tissues. (E-F) Levels of myeloperoxidase (MPO) (E) and matrix metalloproteinase-9 (MMP-9) (F) in bronchoalveolar lavage fluid (BALF). (G-I) (G) Representative transmission electron microscopy (TEM) images of lung tissues (scale bar: 2 μm). (H) Proportion of damaged mitochondria. (I) Average number of mitophagic events per cell. (J) Immunofluorescence co-localization of mitochondrial marker TOMM20 (red) and EpCAM (green) in lung tissues (nuclei stained with DAPI, scale bar: 50 μm). (K-L) Protein expression levels of Beclin-1 (K) and LC3 (L) in lung tissues, with insets showing immunofluorescence co-localization of Beclin-1 (green) and LC3 (red) across treatment groups (nuclei stained with DAPI, scale bar: 50 μm). ∗ vs. Sham; # vs. IRI; & vs. IRI + PD-L1@nmEVs, p < 0.05.

    Article Snippet: For detecting neutrophil characteristic proteins, the primary antibodies comprised CD11b (HA722075, HUABIO, China), CXCR2 (ER1906-87, HUABIO, China), RAGE (ET1702-27, HUABIO, China), TLR2 (ET1705-92, HUABIO, China), TNFR1(RT1624, HUABIO, China), IL6R ( AWA43078 , Abiowell, China) and the exosome marker CD63 (ET1607-2, HUABIO, China).

    Techniques: Activation Assay, Immunofluorescence, Expressing, Transmission Assay, Electron Microscopy, Marker, Staining