cd11b (Miltenyi Biotec)
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Miltenyi Biotec
cd11b
Cd11b, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b/CD11b+Antibody%2C+anti-mouse%2C+REAfinity/pmc13694607-279-9-13
Average 95 stars, based on 238 article reviews
Cd11b, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b/CD11b+Antibody%2C+anti-mouse%2C+REAfinity/pmc13694607-279-9-13
Average 95 stars, based on 238 article reviews
cd11b - by Bioz Stars,
2026-11
95/100 stars
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Magnetic Cell Separation:Article Title: SiglecF-Expressing Neutrophils Differentiate from Mature Neutrophils Locally in the Heart after Myocardial Infarction. Article Snippet: .. The cell pellet was resuspended in 1 mL of ice-cold MACS Rinsing Buffer (Miltenyi Biotec) containing fluorescently labeled antibodies specific for Article Title: XIAOSHUI formula inhibits malignant pleural effusion by targeting the STC1/p65/CXCL5 axis to reprogram tumor-associated macrophages. Article Snippet: Background: Malignant pleural effusion (MPE) is a common and debilitating complication in advanced lung carcinoma, driven by an immunosuppressive tumor microenvironment (TME), where tumor-associated macrophages (TAMs) play a pivotal role.. This specialized milieu not only facilitates neoplastic proliferation but also contributes to immune system dysfunction.. Preliminary evidence suggests that Xiaoshui Formula (XSF) could inhibit MPE by modulating TAM polarization, a key mechanism underlying T cell dysfunction and compromised tumor surveillance capabilities. Labeling:Article Title: SiglecF-Expressing Neutrophils Differentiate from Mature Neutrophils Locally in the Heart after Myocardial Infarction. Article Snippet: .. The cell pellet was resuspended in 1 mL of ice-cold MACS Rinsing Buffer (Miltenyi Biotec) containing fluorescently labeled antibodies specific for other:Article Title: Trimethylamine N -Oxide, a Bariatric Surgery-Associated Host-Microbial Cometabolite, Reduces Colonic Tumorigenesis in a Sex-Dependent Manner Article Snippet: CD11b , M1/70.15.11.5 , n/a (dilution 1:800) , Article Title: Tumor microenvironment remodeling by STING agonism sensitizes endothelial cells to cytotoxic anti-PD-L1/L2 antibody. Article Snippet: B16-PD-L2 or TS/A tumors were harvested, finely minced with sterile blades, and digested at 37 °C for 30 min on an orbital shaker in RPMI-1640 medium containing collagenase I and IV (1 mg/mL each; Merck). Flow Cytometry:Article Title: A novel Chimeric Antigen Receptor (CAR) - strategy to target EGFR VIII -mutated glioblastoma cells via macrophages Article Snippet: After culture for 48 h at 37 °C, 5% CO 2 , adherent cells were detached using StemProTM AccutaseTM (Gibco, Cat#: A1110501) for 10 min and analyzed by FACS to evaluate the phenotypic markers of M1 or M2 macrophages. .. Flow cytometry was performed by using classical myeloid markers such as FACS:Article Title: Maternal Obesity Programs Adipogenic Commitment in Neonatal Mesenchymal Stem Cells: A Link to Redox‐Dependent FOXO1 Signaling Article Snippet: .. Surface markers were characterized according to the International Society of Cell Therapy criteria (Dominici et al. 2006) Briefly, cells were detached with trypsin and centrifuged at 2500 g for 3 min. After this, 1 × 10 6 cells were resuspended in 100 μL of FACS buffer (2% FCS in DPBS) in FACS tubes (Falcon, Corning) and incubated for 30 min in the dark with antibodies for CD73 (APC, 1:50, Miltenyi Biotec), CD90 (PE, 1:50, Miltenyi Biotec), CD105 (BV421, 1:20, BioLegend), CD34 (PE‐Dazzle 594, 1:20, Biolegend), CD45 (BV785, 1:20, BioLegend), and Incubation:Article Title: Maternal Obesity Programs Adipogenic Commitment in Neonatal Mesenchymal Stem Cells: A Link to Redox‐Dependent FOXO1 Signaling Article Snippet: .. Surface markers were characterized according to the International Society of Cell Therapy criteria (Dominici et al. 2006) Briefly, cells were detached with trypsin and centrifuged at 2500 g for 3 min. After this, 1 × 10 6 cells were resuspended in 100 μL of FACS buffer (2% FCS in DPBS) in FACS tubes (Falcon, Corning) and incubated for 30 min in the dark with antibodies for CD73 (APC, 1:50, Miltenyi Biotec), CD90 (PE, 1:50, Miltenyi Biotec), CD105 (BV421, 1:20, BioLegend), CD34 (PE‐Dazzle 594, 1:20, Biolegend), CD45 (BV785, 1:20, BioLegend), and Centrifugation:Article Title: XIAOSHUI formula inhibits malignant pleural effusion by targeting the STC1/p65/CXCL5 axis to reprogram tumor-associated macrophages. Article Snippet: Background: Malignant pleural effusion (MPE) is a common and debilitating complication in advanced lung carcinoma, driven by an immunosuppressive tumor microenvironment (TME), where tumor-associated macrophages (TAMs) play a pivotal role.. This specialized milieu not only facilitates neoplastic proliferation but also contributes to immune system dysfunction.. Preliminary evidence suggests that Xiaoshui Formula (XSF) could inhibit MPE by modulating TAM polarization, a key mechanism underlying T cell dysfunction and compromised tumor surveillance capabilities. Purification:Article Title: XIAOSHUI formula inhibits malignant pleural effusion by targeting the STC1/p65/CXCL5 axis to reprogram tumor-associated macrophages. Article Snippet: Background: Malignant pleural effusion (MPE) is a common and debilitating complication in advanced lung carcinoma, driven by an immunosuppressive tumor microenvironment (TME), where tumor-associated macrophages (TAMs) play a pivotal role.. This specialized milieu not only facilitates neoplastic proliferation but also contributes to immune system dysfunction.. Preliminary evidence suggests that Xiaoshui Formula (XSF) could inhibit MPE by modulating TAM polarization, a key mechanism underlying T cell dysfunction and compromised tumor surveillance capabilities. Mass Spectrometry:Article Title: XIAOSHUI formula inhibits malignant pleural effusion by targeting the STC1/p65/CXCL5 axis to reprogram tumor-associated macrophages. Article Snippet: Background: Malignant pleural effusion (MPE) is a common and debilitating complication in advanced lung carcinoma, driven by an immunosuppressive tumor microenvironment (TME), where tumor-associated macrophages (TAMs) play a pivotal role.. This specialized milieu not only facilitates neoplastic proliferation but also contributes to immune system dysfunction.. Preliminary evidence suggests that Xiaoshui Formula (XSF) could inhibit MPE by modulating TAM polarization, a key mechanism underlying T cell dysfunction and compromised tumor surveillance capabilities. Immune Cell:Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis Article Snippet: For intracellular staining, cells were fixed and permeabilized using the Inside Stain kit according to the manufacturer’s instructions (Miltenyi Biotec) before intracellular antibody staining. .. The following antibodies were used for immune cell characterization: Immunopeptidomics:Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis Article Snippet: For intracellular staining, cells were fixed and permeabilized using the Inside Stain kit according to the manufacturer’s instructions (Miltenyi Biotec) before intracellular antibody staining. .. The following antibodies were used for immune cell characterization: Isolation:Article Title: Increased Constitutive Interferon-β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein. Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8+ , CD11b+ and CD11c+ cells with TRI reagent (Sigma-Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse-transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8+ , Selection:Article Title: Increased Constitutive Interferon-β Levels and Altered CD4 T Cell Homeostasis Induced by Expression of a Viral Glycoprotein. Article Snippet: Total RNA was extracted from entire organs (spleen/thymus) and from isolated splenic CD8+ , CD11b+ and CD11c+ cells with TRI reagent (Sigma-Aldrich), treated with TURBO DNase (Thermo Fisher), and reverse-transcribed with SuperScript IV Reverse Transcriptase (Thermo Fisher) following the instructions of the manufacturers. .. CD8+ , |
